HEK 293T Landing Pad Dose Response Curves

Positive selection for recombined selection is a pretty useful technique when working with the HEK 293T landing pads, but people might not know what concentrations are best. Well, here is that info, all in one place. In each case, the antibiotic resistance gene had been placed after an IRES element in an attB recombination plasmid, and was linked to mCherry using a 2A stop-start sequence. Thus, the Y axis is showing how well the mCherry+ cells were enriched at various concentrations of antibiotic.

Consistent with the above plots, I generally suggest people use 1 ug/mL Puro, 10 ug/mL Blast, 100 ug/mL Hygro, and 100 ug/mL Zeocin for selections.