Terrific for lentivector growth?

At some point, I was chatting with Melissa Chiasson about plasmid DNA yields, and she mentioned that her current boss had suggested using terrific broth instead of Luria broth for growing transformed bacteria. I think both of us were skeptical at first, but she later shared data with me showing that DNA from e.coli grown in TB had actually given her better yield. I thus decided it was worth trying myself to see if I could reproduce it in my lab.

There are two general types of plasmids we tend to propagate a lot in my lab. attB recombination vectors, for expressing transgenes within the landing pad, and also lentiviral vectors of the “pLenti” variety, which play a number of different roles including new landing pad cell line generation and pseudovirus reporter assays.

I first did side-by-side preps of the same attB plasmids grown in TB or LB, and TB-grown cultures yielded attB plasmid DNA concentrations that were slightly, albeit consistently worse. But I eventually I tested some lentiviral vector plasmids and finally saw the increase in yield from TB that I had been hoping for. Relaying this to Melissa, she noted she had been doing transformations with (presumably unrelated sets of) lentiviral vectors herself, so these observations had been consistent after all.

Thus, if you get any attB or pLenti plasmids from me, you should probably grow them in LB (attB plasmids) and TB (pLenti plasmids), respectively, to maximize the amount of DNA yields you get back for your efforts.