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Virus-Host Amino Acid Compatibilities (eg. During Viral Entry)

Virus-Host Amino Acid Compatibilities (eg. During Viral Entry)

Posted on July 27, 2020August 18, 2022 by kmatreyek
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Cell Engineering

Cell Engineering

Posted on August 12, 2019August 18, 2022 by kmatreyek
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Clinical Genetics and Inborn Errors of Immunity

Clinical Genetics and Inborn Errors of Immunity

Posted on August 12, 2019August 18, 2022 by kmatreyek
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Mammalian Directed Evolution

Mammalian Directed Evolution

Posted on May 18, 2019September 28, 2022 by kmatreyek
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Additional entries

  • Barcoding the epilepsy vector
  • Plasmidsaurus decision tree
  • Pymol figures
  • When iCasp9 doesn’t kill
  • Spec comparisons
  • HEK cell small molecule toxicities
  • Quantitating DNA via the plate reader
  • Quantitating cells via the plate reader
  • Lentivector supe collection
  • Trimming and tabulating fastq reads
  • Nanopore denovo assembly
  • Plasmidsaurus fasta standardizer
  • Flow cytometry compensation
  • Common Plasmidsaurus Errors
  • Ordering oligos at CWRU
  • Consistent Plasmidsaurus sequencing miscalls
  • Command line BLAST
  • ODs on the spec and nanodrop
  • Setting up a hybrid lab meeting
  • Designing Amplicon-EZ primers
  • Illumina Library Mixing
  • Generating new indices for multiplex Illumina sequencing
  • Posters
  • Inverse PCR SSM Primer Python Script
  • NSF GRFP at CWRU
  • Using Inkscape
  • Simulating Sampling during Recombination
  • Dual index on Miseq and Nextseq
  • Estimating coverage for NNK SSM transformations
  • HEK 293T Bxb1 Landing Pad recombination protocol
  • Conda virtual environments
  • Analyzing Illumina Fastq data
  • Dealing with paired fastq reads
  • Landing pad plasmid maps
  • Chemical transformation efficiencies
  • HEK 293T Landing Pad Dose Response Curves
  • Nucleotide biases in primers
  • Quantitating Western blots
  • Terrific for lentivector growth?
  • Using SpliceAI for synthetic constructs
  • Shuttling one ORF into another
  • Illumina sequencing of barcoded amplicons from the landing pad
  • Bleed-over on the Nikon scope
  • Pymol Basic Tutorial
  • Ordering primers
  • Setting up a shared Github repo for an RStudio project
  • Pseudovirus Infection Assays
  • Sanger seq analysis – finding correct clones
  • Flowjo Analysis of GFP positive cells
  • Optimal Laser and Detector Filter Combinations for Fluorescent Proteins
  • Designing Primers for Targeted Mutagenesis
  • Using prior data to optimize the future
  • Modeling bacterial growth
  • Awesome labrat youtube videos
  • Gibson / IVA success rates
  • Primer Inventory Google Sheet To Benchling
  • Basic data analysis in R Studio
  • Writing a simple Python script (for Biologists)
  • Installing Enrich2
  • Downloading JoVE videos
  • Simulating sampling during routine lab procedures
  • Software to download
  • Make a movie of a pymol structure spinning for powerpoint
  • VAMP-seq Tips
  • Landing Pad Cell Line Generation
  • Gene knockouts by exon deletion with Cas9 transfection

    • Virus-Host Amino Acid Compatibilities (eg. During Viral Entry)
    • Cell Engineering
    • Clinical Genetics and Inborn Errors of Immunity
    • Mammalian Directed Evolution
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